Kajian Subkronik 28 Hari Ekstrak Metanolik Scurrula atropurpurea terhadap Kadar Creatine Phosphokinase Tikus Betina A Subcronic 28 Days Study of Metanolic Extract Scurrula atropurpurea to Creatine Phosphokinase Levels of Female Rats
Article Sidebar
Main Article Content
Abstract
Tea parasite plants (Scurrula atropurpurea Bl. Dans.) are parasitic plants that live disrupt in tea plant. Scurrula atropurpurea Bl. Dans have many active compound content one of them is Flovonoid like quersetin which can be recovered various diseases. CPK enzyme is one of the clinical biochemical parameters was used determine the safety of test preparations, especially in subcronic in test heart condition, because the enzyme CPK as cardiag biomarker. The purpose this study was to determine the level of CPK in female wistar rats (Rattus novergicus) after being exposed Metanolic Extract Scurrula atropurpurea Bl Dans. (MESA) is subchronically 28 days. This research is True Experimental Design with Completely Randomized. The rats be used were 20 with female genitals were divided into 4 Treatments with Treatment1 control and three Treatments were exposed to EMSA with doses 250, 500, and 1000 mg / KgBW 5 times a week. After 28 days rats be dissected and analyzed levels of CPK enzymes, then the data obtained were analyzed using spss14. It used ANOVA test. ANOVA test results showed that the CPK content of treatment was not significantly different from the controls because the sig value 0.449 more than 0.005 this means that EMSA did not affect the serum CPK enzyme level of female rats and no toxic effect was caused so that EMSA was declared safe by sub-chronic test . The value of the CPK enzyme level of treatment was approaching the control value is 250 Mg / KgBW dose so we recommend the dose.
ABSTRAK
Tanaman benalu teh (Scurrula atropurpurea Bl. Dans.) merupakan tanaman parasit yang hidup mengganggu pada tanaman teh. Scurrula atropurpurea Bl. Dans memiliki banyak kandungan senyawa aktif salah satunya yaitu Flovonoid seperti quersetin yang dapat menyembuhkan berbagai penyakit. Enzim Creatine Phosphokinase (CPK) merupakan salah satu parameter biokimia klinis yang digunakan untuk mengetahui keamanan sediaan uji terutama dalam uji subkronik terutama pada keadaan jantung, karena enzim CPK sebagai cardiag biomarker. Tujuan penelitian ini adalah untuk mengetahui kadar CPK pada tikus wistar (Rattus novergicus) betina setelah dipapari Ekstrak Metanolik Scurrula atropurpurea Bl Dans. (EMSA) secara sub-kronik selama 28 hari. Penelitian ini adalah True Experimental Design dengan Rancangan Acak Lengkap. Tikus yang digunakan ada 20 ekor dengan kelamin betina dibagi menjadi 4 perlakuan dengan perlakuan I sebagai kontrol dan tiga perlakuan dipapari EMSA dengan dosis 250, 500, dan 1000 mg/KgBB 5 kali seminggu. Setalah 28 hari tikus dibedah dan dianalisis kadar enzim CPK, selanjutnya data yang didapat dianalisis menggunakan spss14. Menggunakan uji ANOVA. Hasil analisis uji ANOVA menunjukkan bahwasanya kadar CPK perlakuan tidak berbeda nyata dengan kontrol karena nilai sig 0,449 lebih dari 0,05 hal ini berarti EMSA tidak berpengaruh terhadap kadar enzim CPK serum tikus betina dan tidak ada efek toksik yang ditimbulkan sehingga EMSA dinyatakan aman secara uji sub-kronik. Nilai kadar enzim CPK perlakuan yang mendekati nilai kontrol yaitu dosis 250 mg/KgBB sehingga kami merekomendasikan dosis tersebut.
Kata kunci : EMSA, CPK, Toksisitas Sub-kronik.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Attribution:
Jurnal Biosaintropis (Bioscience-Tropici s licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC-BY). The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to automatic transfer of the copyright to the publisher.
Permissions:
Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from one of the authors.
Ethical matters:
Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the authors™ own must have had the correct authorization for this exportation.
References
[2]Athiroh, N, AS. 2017. Monograf Benalu Teh Dan Hipertensi. Intelegensia Media. ISBN: 978-602-6874-85-6. Malang.
[3]Athiroh, N., Widodo MA, dan Widjajanto E. 2000. Efek Scurrula Oortiana (Benalu Teh) dan Macrosolen javanus (Benalu Jambu Mawar) Terhadap Kontraktilitas Pembuluh Darah Arteri Ekor Tikus Terpisah Dengan Atau Tanpa Endotel. Tesis. Malang: UniversitasBrawijaya.
[4]Athiroh, N. Kontraktilitas Pembuluh Darah Arteri Ekor Terpisah Dengan Atau TanpaEndotel Setelah Pemberian Ekstrak Scurrula oortiana (Benalu Teh). Jurnal Berkala Hayati Edisi Khusus 3D. 2009: 31-34.
[5]Athiroh, N., and E. Sulistyowati. 2013. Scurrula atropurpurea Increases Nitric Oxide and Decreases Malondialdehyde in Hypertensive Rats. Jurnal Universa Medicina. 32 (1) : 44-50.
[6]Athiroh, N., N. Permatasari, D. Sargawo dan M.A Widodo. 2014. Antioxidative and Blood Pressure-Lowering Effects of Scurrula atropurpurea On Deoxycorticosterone Acetate-Salt Hypertensive Rats. Biomarkers and Genomic Medicine. 6 (1): 32-36.
[7]Athiroh, N., Permatasari, D. Sargowo dan M.A. Widodo. 2014. Effect of Scurrula atropurpurea on Nitric Oxide, Endothelial Damage, and Endothelial Progenitor Cells of DOCA- salt Hypertensive rats. Iranian Journal of Basic Medical Sciences. 17 (8): 622-625.
[8]Athiroh, N., and Wahyuningsih, D. 2017. Study of Superoxide Dismutase and Malondialdehyde Concentrations in Mice After Administration of Methanolic Extract of Scurrula atropurpurea (Bl.) Dans. Jurnal Kedokteran Hewan. 11 (1) : 19-22.
[9]Hikmah, U. Athiroh, N., dan Santoso, H. 2017. Kajian Ektrak Metanolik Scurrula atropurpurea (Bl.) Dans. Terhadap Serum Glutamic Oxaloacetic Transaminase Tikus Betina. e-urnalIlmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). 2 (2): 30-35.
[10]Athiroh, N dan Permatasari, N. 2011. Mekanisme Deoxycorticostreone acetate (DOCA)- garam terhadap Peningkatan Tekanan Darah pada Hewaan Coba. Jurnal Biologi el- Hayah.1 (4): 1-4.
[11]Munawaroh, N.S. Athiroh, N., dan Santoso, H. 2016. Kajian Ektrak Metanolik Scurrulaatropurpurea (Bl.) Dans. Terhadap Kadar Trigliserida Tikus Betina. e-Jurnal IlmiahBIOSAINTROPIS (BIOSCIENCE-TROPIC). 2 (1): 59-64.
[12]Zakia, J. F. dan Athiroh, N. 2017. Studi Sub-Kronik 90 Hari: Pengaruh Ekstrak Scurrula atropurpurea Terhadap Kadar Kolesterol Tikus (Rattus norvegicus) Betina Galur Wistar. e-JurnalIlmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). 3 (2): 37 – 43.
[13]Fajrin, S. A dan Athiroh, N. 2017. Studi Kadar Lipid Trigliserida pada Tikus Wistar Setelah Pemberian Ekstrak Metanolik Scurrula atropurpurea (Bl.) Dans Secara Subkronik Selama 90 Hari. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). 3 (2): 24 – 29.
[14]Prastika, N.I., Athiroh, N., Santoso, H. 2017. Pengaruh Pemberian Subkronik Ekstrak Metanolik Scurrula atropurpurea (Bl) Dans Terhadap Kadar Kreatinin Tikus Wistar. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). 2 (2): 42 – 48.
[15]Zahroh, D.F., Athiroh, N., Santoso, H. 2017. Efek Pemberian Ektrak Metanolik Scurrula atropurpurea (Bl) Dans Terhadap Kadar Kolesterol Tikus Wistar Secara Subkronik. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). 3 (1): 8 – 14.
[16]Fatima, H., Athiroh, N., Santoso, H. 2017. Pemberian Ekstrak Metanolik Scurulla atropurpurea (Bl) Dans Secara Subkronik Terhadap Protein Total Dan Albumin Tikus Betina. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). 2 (2): 49 – 54.
[17]Mihmidati, L dan Athiroh, N. 2017. Pengaruh Ekstrak Metanolik (Scurrula atropurpurea (Bl.) Dans) yang Diberikan Secara Subkronik 90 Hari Pada Tikus Betina (Rattus norvegicus) Terhadap Necrosis Otak. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). 3 (2): 16 – 23.
[18]Fahmiy, R dan Athiroh, N. 2017. Studi Sub-Kronik 90 Hari: Profil Histopatologi Pada Jaringan Pulmo Tikus (Rattus norvegicus) Akibat Paparan Ekstrak Scurrula atropurpurea (Bl.) Dans. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). 3 (1): 39 – 45.
[19]Hidayati, D.M.N dan Athiroh, N. 2017. Profil Histopatologi pada Jaringan Jantung Tikus Subkronik 90 Hari Menggunakan Ekstrak Metanolik Scurrula atropurpurea (Bl.) Dans. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). 3 (2): 30 – 36.
[20]Harjono P, Y. 2004. Hubungan Antara Kerusakan Otak Pada Stroke Akut Dengan Peningkatan Cretine Phosphokinse. Tesis. Ilmu Penyakit Saraf FK UNDIP. Semarang.
[21]Athiroh, N., Sulistyowati E. 2015. Evalution of Methanolic Extract of Scurrula atropurpurea (BI.) Dans Sub-Chronic Exposure on Wistar Rat Liver. AENSI Journal. ISSN-1995-0756.
[22]BPOM. 2014. Pedoman Uji Toksisitas NonKlinik Secara in vivo. Peraturan Badan Pengawas Obat dan Makanan Republik Indonesia. Indonesia.
[23]DiaSys. 2009. Diagnostic System GmbH. Holzheim. Alte Strasse. 9 65558. Germany.
[24]Solikin. 2016.Upaya Perbanyakan Generatif Benalu: Dendrophthoe pentandra (L.)Miq. International Conference on Global Resource Conversation. Pasuruan
[25]Yi Ma, Zhao Yicen, Walker K, Robin and Berkowiltz A Gerald. 2013.Molecular Steps in the Immune Signaling Pathway Evoked by Plant Elicitor Peptides : Ca2+ Dependent Protein Kinase, Nitric Oxide, and Reactiv , Oxygen Species Are Downstream from the Early Ca2+ Signal. Plant Physiologi. 163: 1459-1471.
[26]Burtis AC, Ashwood ER. 1996. Enzyme. In : Tietz fundamentals of clinical chemistry. WB Saunders Company. Philadelphia.: 283-313.
[27]Brewster, Lizzy,. Gideon, Navin, R., Bindraban, MD. Richard, P., Koopmans, Joseph, F. Clark, PhD. Gert, A. Van. 2006Creatine Kinase Activity Is Associated With Blood Pressure. Hypertension.. DOI: 10.1161/CIRCULATIONAHA. 105.584490. URL:http://www.circulationaha.org