Kadar Creatine Kinase Myocardial Band Pada Tikus Wistar Betina Yang Dipapar Ekstrak Metanolik Scurrula atropurpurea Subkronik 28 Hari Creatine Kinase Myocardial Band Level in Female Wistar Rats Exposed to Metanolic Extract Scurrula atropurpurea on Subchronic 28 Days
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Abstract
Scurrula atropurpurea (Bl.) Dans is a parasitic plant that lives on the tea plant (Tea sinensis L.) and it is very potential as medicine. The purpose of this study was to determine the level Creatine Kinase Myocardial Band (CK-MB) in female wistar rats after giving Metanolic extract Scurrula atropurpurea (MESA) for 28 days (subchronic exposure). The type of this research is True Experimental Design with Completely Randomized Design (CRD). The number of animal research was 20 female wistar rats weighing 100-200 g. Then divide into 4 treatments; control, MESA dose 250 mg/KgBW, 500 mg/KgBW, 1000 mg/KgBW. MESA is given 5 times a week for 28 days orally. After 28 days the rats were dissected and done the examination of CK-MB levels from the blood serum, the result of CK-MB levels of the treatments were compared with the controls. The result showed that the CK-MB levels in the rats treated serum 250, 500, 1000 mg/KgBW was decreased when compared with controls, and after the One-way ANOVA test showed that the treatments were not significantly different then compared with controls. In this case showed that giving MESA to with female wistar rats (Rattus norvegicus) is not toxic, so MESA is safe by subchronic test.
Keyword: MESA, CK-MB, Subkronik
ABSTRAK
Scurrula atropurpurea (Bl.) Dans merupakan tanaman parasit yang hidup menumpang pada tanaman teh (Tea sinensis L.) dan sangat berpotensi sebagai obat obatan. Tujuan Penelitian ini adalah untuk mengetahui kadar Creatine Kinase Myocardial Band (CK-MB) pada tikus wistar betina setelah pemberian Ekstrak Metanolik Scurrula atropurpurea (EMSA) selama 28 hari (paparan subkronik). Jenis penelitian ini merupakan True Experimental Design dengan Rancangan Acak Lengkap (RAL). Jumlah hewan uji adalah 20 ekor tikus wistar betina dengan berat badan berkisar 100-200 g, kemudian dibagi menjadi 4 perlakuan yaitu kontrol, EMSA dosis 250 mg/KgBB, 500 mg/KgBB, 1000 mg/KgBB. EMSA diberikan 5 kali dalam seminggu selama 28 hari secara oral. Setelah 28 hari tikus di bedah dan dilakukan pemeriksaan kadar CK-MB dari serum darah, hasil kadar CK-MB pada perlakuan dibandingkan dengan kontrol. Hasil penelitian menunjukkan bahwa kadar CK-MB pada serum tikus perlakuan 250, 500, 1000 mg/KgBB mengalami penurunan jika dibandingkan dengan kontrol, dan setelah di uji One-way ANOVA menunjukkan bahwa pada perlakuan tidak berbeda nyata jika dibandingkan dengan kontrol. Hal ini menunjukkan bahwa dengan pemberian EMSA pada tikus putih (Rattus norvegicus) wistar betina adalah tidak toksik sehingga EMSA aman secara uji subkronik.
Kata Kunci : EMSA, CK-MB, Subkronik
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References
[2] Athiroh, N dan Permatasari, N. 2012. Mekanisme Kerja Benalu Teh pada Pembuluh Darah. Jurnal Kedokteran Brawijaya. 27 (1) : 1-4.
[3] Athiroh, N. 2009. Kontraktilitas Pembuluh Darah Arteri Ekor Tikus Terpisah dengan atau Tanpa Endotel setelah Pemberian Ekstrak Scurrula oortiana (Benalu Teh). Jurnal Berkala Hayati Edisi Khusus 3D: 31-34.
[4] Athiroh, N dan Permatasari, N. 2011. Mekanisme Deoxycorticostreone acetate (DOCA)-garam terhadap Peningkatan Tekanan Darah pada Hewan Coba. Jurnal Biologi el-Hayah. 1 (4) : 1-4.
[5] Athiroh, N., Permatasari, N., Sargowo, D., and Widodo, M.A. 2014. Antioxidative and Blood Pressure-Lowering Effects from Scurrula atropurpurea on DOCA-salt Hypertensive Rats. Biomarkers and Genomic Medicine. 6 (1) : 32-36
[6] Athiroh, N., Permatasari, N., Sargowo, D., and Widodo, M.A. 2014. Effect of Scurrula atropurpurea on Nitric Oxide, Endothelial Damage, and Endothelial Progenitor Cells of DOCA-salt Hypertensive Rats. Iranian Journal of Basic Medical Sciences. 17(8):622-625.
[7] Athiroh, N & Sulistyowati, E. 2013. Scurulla atropurpurea Increases Nitric Oxide and Decrease Malondeladehyde in Hypertensive Rats. Jurnal Universa Medicina. 32 (1): 44-50.
[8] Athiroh, N., and Wahyuningsih, D. 2017. Study of Superoxide Dismutase and Malondialdehyde Concentrations in Mice After Administration of Methanolic Extract of Scurrula atropurpurea (Bl.) Dans. Jurnal Kedokteran Hewan, March 2017. 11 (1): 19-22.
[9] Athiroh, N.A.S. 2017. Monograf Benalu Teh dan Hipertensi. Edisi 01. Intelegensia Media. Malang.
[10] Athiroh, N., Sulistyowati. 2015. Evaluation of Methanolic Extract of Scurrula Atropurpurea (Bl.) Dans Sub-Chronic Exposure On Wistar Rat Liver. Advances in Environmental Biology, 9(23) October 2015, Pages: 245-250
[11] Argus, Athiroh, N., dan Santoso, H. 2016. Paparan 28 Hari Ekstrak Metanolik Scurrula atropurpurea (Bl.) Dans. Terhadap Kadar SGPT Tikus Betina. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 2. No:1. Hal: 53-58.
[12] Hikmah, U. Athiroh, N., dan Santoso, H. 2017. Kajian Ektrak Metanolik Scurrula atropurpurea (Bl.) Dans. Terhadap Serum Glutamic Oxaloacetic Transaminase Tikus Betina. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 2. No:2. Hal: 30-35.
[13] Munawaroh, N.S. Athiroh, N., dan Santoso, H. 2016. Kajian Ektrak Metanolik Scurrula atropurpurea (Bl.) Dans. Terhadap Kadar Trigliserida Tikus Betina. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 2. No:1. Hal: 59-64.
[14] Sammad, F.H.A., Athiroh, N., dan Santoso, H. 2017. Pemberin Ektrak Metanolik Scurrula atropurpurea (Bl.) Dans. Secara Subkronik Terhadap Protein Total dan Albumin Tikus Betina. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 2. No:2 Hal: 49-54.
[15] Prastika, N.I, Athiroh, N., dan Santoso, H. 2017. Pengaruh pemberian Subkronik Ektrak Metanolik Scurrula atropurpurea (Bl.) Dans. Terhadap Kadar Kreatinin Tikus Wistar. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 2. No:2. Hal: 42-48.
[16] Zahroh, D. F., Athiroh, N., dan Santoso, H. 2017. Efek Pemberin Ektrak Metanolik Scurrula atropurpurea (Bl.) Dans.Terhadap kadar Kolesterol Tikus Wistar Secara Subkronik. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 3. No:1 Hal: 8-14.
[17] Mihmidatin, L. Athiroh, N., dan Santoso, H. 2017. Metanolic Extraction of (Scurrula atropurpurea (Bl.) Dans) Effect which is given 90-Days Sub-chronic on Female Rats (Rattus norvegicus) toward Necrosis of Brain. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 3. No. 2. Hal. 16 – 23.
[18] Zakia, J. F. Athiroh, N., dan Santoso, H. 2017. Study of Sub Chronic during 90 Days: Effect of Scurrula atropurpurea Extract toward Cholesterol Level in Rat (Rattus norvegicus). e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 3. No. 2. Hal. 37 – 43.
[19] Hidayati, D. M. N. Athiroh, N., dan Santoso, H. 2017. Histopathologic Profile in the Heart Tissue of 90-Days Sub-Chronic Rats Using Metanolic Extract of Scurrula atropurpurea (Bl.) Dans. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 3. No. 2. Hal. 30 – 36.
[20] Fahmiy, R. Athiroh, N., dan Santoso, H. 2017. Study of 90 Days Sub Chronic: The Histopathology Profile in The Pulmo Tissue Rats Exposured Scurrula atropurpurea (Bl.) Dans. Extraction. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 3. No. 1. Hal. 39 – 45.
[21] Fajrin, S. A. Athiroh, N., dan Santoso, H. 2017. Study of Triglyceride Lipid Level in Rats after Sub-cronic Exposure within 90-Days of Methanolic Extract of Scurrula atropurpurea (BI.) Dans. e-Jurnal Ilmiah BIOSAINTROPIS (BIOSCIENCE-TROPIC). Vol. 3. No. 2. Hal. 24 – 29.
[22] BPOM, 2014. Pedoman Uji Toksisitas Nonklinik Secara In Vivo. Jakarta, BPOM
[23] DiaSys. 2009. Diagnostic Systems GmbH Alte Strasse 9 65558 Holzheim Germany.
[24] Ladesman, R. 2012. Pola Biomarker Kreatin Kinase dan CK-MB pada Pasien Infark Miokard Akut diBagian Penyakit Dalam Rumah Sakit Mohammad Hoesin Palembang. Skripsi. Fakultas Kedokteran, Universitas Sriwijaya.
[25] Zakia, J. F. 2017. Study of Sub Chronic during 90 Days: Effect of Scurrula atropurpurea Extract toward Cholesterol Level in Rat (Rattus norvegicus). Skripsi. Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Islam Malang.