METODE PEMBERIAN KOLKISIN TERHADAP RESPON MORFOLOGIS TANAMAN ZAITUN (Olea europeae L.)
Article Sidebar
Main Article Content
Abstract
Olive ( Olea europaea L. ) is a species native to the Mediterranean growing since 4000 BC. Colchicine (C22H25O6N) Represents white alkaloid derived from tubers of plants (Colchichum autumnale L.). These compounds can block the formation of threads spindle in cell division, causing cells can not divide and forming individual polyploidy. Polyploidy are organisms that have more than two sets of chromosomes or genome in the cell somatisnya. This study aims to investigate the response of plant morphological olive (Olea europeae L.) to the method and the concentration of colchicine. Experimental research methods used were designed using a randomized block design (RAK) factorial and consists of two treatment factors, namely droplets, immersion, and the combination of (drip and immersion), the second factor is the concentration of colchicine 0.25%, 0.5%, 0.75%, and 1%. The results showed that the concentration of colchicine effect on plant morphological olive (Olea europeae L.), the best response to the morphological olive crop is the drip method either a drop or two drops on plant height, diameter and number of leaves. The results of the analysis (ANOVA Test) also states that provide significantly different results then performed LSD (Least Significant Differences) to know the difference between methods.
Zaitun (Olea europaea L.) merupakan jenis tanaman asli daerah Mediterania yang tumbuh sejak 4000 SM. Kolkisin (C22H25O6N) Merupakan senyawa alkaloid berwarna putih yang diperoleh dari umbi tanaman (Colchichum autumnale L.). Senyawa ini dapat menghalangi terbentuknya benang-benang spindel pada pembelahan sel, sehingga menyebabkan sel tidak dapat membelah dan terbentuklah individu poliploidi. Poliploidi adalah peristiwa penggandaan krmosom sehingga mempunyai lebih dari dua sel atau genom dalam sel somastisnya. Penelitian ini bertujuan untuk mengetahui respon morfologis tanaman zaitun (Olea europeae L.) terhadap metode dan konsentrasi pemberian kolkisin. Metode penelitian yang digunakan eksperimental yang dirancang dengan menggunakan Rancangan Acak Kelompok (RAK) secara faktorial dan terdiri dari dua faktor perlakuan yaitu tetesan, perendaman, dan kombinasi (tetes dan perendaman), faktor kedua yaitu konsentrasi kolkisin 0,25%, 0,5%, 0,75%, dan 1%. Hasil penelitian menunjukkan bahwa, konsentrasi pemberian kolkisin berpengaruh pada morfologis tanaman zaitun (Olea europeae L.), respon terbaik pada morfologis tanaman zaitun adalah dengan metode tetesan baik satu tetes maupun dua tetes pada tinggi tanaman, diameter, dan jumlah daun. Hasil analisis (Uji Anova) menunjukkan bahwa memberikan hasil berbeda nyata selanjutnya dilakukan uji LSD (Least Significant Differences) untuk mengetahui perbedaan antar metode. Metode tetesan merupakan metode terbaik dalam pemberian kolkisin dilihat dari tinggi tanaman, diameter, dan pertambahan jumlah daun dibanding dengan perendaman, maupun kombinasi (tetes dan perendaman).
Kata Kunci: Zaitun (Olea europeae L.), Kolkisin, Metode.
Article Details
Copyright and Attribution:
Jurnal Biosaintropis (Bioscience-Tropici s licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC-BY). The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to automatic transfer of the copyright to the publisher.
Permissions:
Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from one of the authors.
Ethical matters:
Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the authors™ own must have had the correct authorization for this exportation.
References
[2] USITC (United States International Trade Comission (US). 2013. Olive Oil Condition of Competition between US and Major Foreign Supplier.). USITC. Washington.
[3] Foster, R, Lunn J, Williamson CS. 2009. Culinary oils and their health effects. J. Br Nutr Bull 34: 4-47.
[4] FDA (Food and Drug Administration) (US). 2004. Fda allows qualified health claim to decrease risk of coronary health disease. Diterima 19 Mei 2016. URL:http//www.fda.gov/newsevents/ newsroom/pressannouncements/2004/ucm108368.htm.
[5] Iqbal, M, 2013. Zaitun Indonesia Incorporated. Artikel ilmiah Gerai dinar. Akses tanggal 18 April 2016. URL:http://graidinar.com/index.php/using-joomla/extensions/components/content-component/article-categories/81-gd-articles/enterpreneurship/1367-zaitun-indonesia-incorporated.
[6] Suryo, 1995. Sitogenetika.2. Gadjah Mada University Press. Yogyakarta. 17-226
[7] Burns, G.W., 1972. The Science Of Genetics, an Introduction to Heredity, 2nd Edition.The Macamillan Company. New York.
[8] Eigsti, O.J. and P. Dustin Jr.1957. Colchicine in Agriculture, Medicine, Biology and Cemistery.The Lowa State Collage Press. United State Of America.
[9] Poespodarsono, S. 1998. Dasar-dasar Ilmu Pemulian Tanaman. Sumber Infomasi ITB. Bogor.
[10] Rugini E, Fedeli E,. 1990. Legumes and Oilseed I: Olive (Olea europaea L.) as an Oilseed Crop. Bajaj YPS, editor. Springer. New York.
[11] Salisbury, F.B. and C.W. Ross 1995. Fisiologi Tumbuhan (Diterjemakan oleh D.R. Lukman dan Sumaryono). ITB. Bandung 767 Hal.