Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)

Keanekaragaman Bakteri di Pasar Modern Dinoyo Terpadu (MDT) Kota Malang

Ari Prabawati Pangestu, Ahmad Syauqi, Saimul Laili — Sat, 31 Jan 2026 00:00:00 +0000

Air quality is influenced by the diversity of bioaerosol in the circulation system in a room. Bacteria can have a positive effect on human life, but can also have a negative effect. The negative effects caused by bacteria are mostly due to contamination from pathogenic bacteria. Pathogenic bacteria in the air include; Salmonella sp, Staphylococcus aureus, Eschericia coli, Streptococcus pneumoniae, Shigella sp, and Clostridium sp. Pasar Modern Dinoyo Terpadu Blok C is a part of the market which is crowded with visitors, where it sells wet food ingredients such as various types of fish, meat, etc. The condition of the building is classified as good, but the sanitation activities at that time are still not well maintained.The purpose of this study was to determine the pathogenic microorganisms in the air of the Dinoyo Terpadu Modern Market Malang and to determine the differences in the diversity index of microorganism colonies from the three observations made. The research method used survey and descriptive methods by conducting analysis in the laboratory. Colony counter colony count. The calculation of the bacterial colony diversity index using the calculation formula by Shannon-Wiener. The results of the identification of pathogenic bacteria in the air are colonies of cultured bacteria that have characteristics similar to pathogenic bacteria Staphylococcus aureus, Eschericia coli, and Shigella sp.The calculation of the diversity index for the three observations made was not different because the three index values H '<1, which means that the diversity index at that place was low.This can be affected by the air ventilation conditions in the place as well as the temperature and humidity conditions in the sampling area.

Karakteristik Penderita Kanker Payudara Rawat Inap di Kota Malang Tahun 2018

Abel Nabila Anbarwati, Nour Athiroh Abdoes Sjakoer, Ratna Djuniwati Lisminingsih — Sat, 31 Jan 2026 00:00:00 +0000

Breast cancer is a malignant tumor that occurs and strikes the tissue of the breast and it is the second highest cause of death that commonly found in women in Indonesia. Malang is a city located in East Java Province which has the highest indication of breast cancer prevalence in 2018, namely (2,5%) out of 5.326 who have conducted symptomatic examinations (Investigation to the city health dapertment data is collected by poor health and hospital in the town of Malang in January-descember). Data from the health agency poor city the year 2018. Breast cancer cases often occur in women every year, there is either a decrease or an increase in breast cancer cases in Malang city. The research is qualitative research descriptive. The sample collection is done purposively sampling, and obtained 14 respondents from the test were made and intended for breast cancer patients. The results showed that the acquisition of a characteristic description of the incidence of breast cancer cases in Malang were age (p- 30-39 years = 71,5%), eccupation (p- private employees = 35,7%), education (p- SMA = 57,1%), menarche status (p- <11 years = 64,2%), menopausal status (p- >55 years = 21,4%), and breastfeeding activity (p- breastfeeding = 21,5%).

Proporsi Fenotip dan Frekuensi Alel Golongan Darah Sistem ABO dan Rhesus pada Populasi Etnis Arab di Kampung Arab Bondowoso

Eka Safitri Nur Aini, Rike Oktarianti, Mahriani, Asmoro Lelono — Sat, 31 Jan 2026 00:00:00 +0000

Knowledge of the ABO and Rhesus blood group systems plays an essential role in blood transfusion practices, population genetics studies, and the resolution of forensic cases such as paternity disputes. This study aimed to determine the phenotypic proportions and allele frequencies of the ABO and Rhesus blood group systems in the Arab ethnic population residing in Kampung Arab, Bondowoso Regency. The research samples were collected randomly using a lottery technique, involving a total of 315 participants. Blood group identification was performed using the slide test method, which is based on agglutination reactions between antigens (agglutinogens) and antibodies (agglutinins). determination of blood group was carried out by the antigen-antibody agglutination test. The results showed the phenotypic distribution of blood groups as follows: A+ (31.13%), B+ (25.68%), O+ (38.91%), and AB+ (4.28%). The allele frequencies for the ABO system were IA (0.223), IB (0.143), and i (0.634), while the frequency of the positive Rhesus allele (D) was 1. This study provides an overview of the distribution patterns of blood groups and genetic variation within the Arab ethnic community in Bondowoso, which may serve as a foundation for future population genetics research and medical applications.

Pengaruh Berbagai Tingkat pH pada Media MS Cair dan Penambahan Nanobubbles (NBS) Nitrogen Terhadap Pertumbuhan Anggrek Dendrobium sp.

Della Nur Hayati, Tintrim Rahayu, Gatra Ervi Jayanti — Sat, 31 Jan 2026 00:00:00 +0000

Orchids (Orchidaceae) are one of the ornamental plants that have aesthetic value and attract attention to be cultivated. Tissue culture is one alternative as orchid propagation with planting media which is influenced by factors, one of which is pH. Recently the technology of nanobubbles (NBs) is used on orchid plants. NBs are the latest nanotechnologies that have been implemented in various fields, such as health, water, agriculture and others. NBs utilize gas bubbles measuring less than 100 nm in diameter. This study aims to determine the effect of nitrogen nanobubbles (NBsN₂) for the growth of Dendrobium sp. orchids. The method applied is to add NBsN₂ to Dendrobium sp. orchids. (aged 3-4 months) with different pH levels, for 35 days after planting (HST) with observation every 7 HST. In this study, the plantlets used had a size of 1 cm, consisting of 2 leaves and had no roots. The results showed that the growth of orchid plants Dendrobium sp. highest at pH treatment 5,4 + 2 ml MS with bud length (0,80 cm), number of buds (1,67 buds) and root length (0,73 cm). Leaf length growth affects the pH treatment of 5,6 + 2 mL with a length of 1,53 cm. The pH and NBsN₂ treatments respond to leaf length, number of shoots and shoot length.

Keywords: Dendrobium, NBs, Nitrogen, Growth, pH

Evaluasi Aktivitas Antibakteri Foot Sanitizer Spray Minyak Atsiri Sereh Wangi (Cymbopogon citratus sp.) terhadap Staphylococcus aureus

Prihastini Setyo Wulandari, Rifqi Ferry Balfas, Fika Rizqia — Sat, 31 Jan 2026 00:00:00 +0000

Foot cleansing spray is a topical preparation used to maintain foot hygiene and prevent the growth of odor-causing bacteria, one of which is Staphylococcus aureus. Lemongrass essential oil (Cymbopogon citratus sp.) is known to have antibacterial activity and has the potential to be used in topical formulations. This study aimed to evaluate the antibacterial activity of foot cleansing spray containing lemongrass essential oil against Staphylococcus aureus. The essential oil was obtained by steam distillation with microwave pretreatment and subsequently formulated into foot cleansing sprays containing 1%, 2%, and 3% essential oil. Antibacterial activity was assessed using the agar diffusion method, with 1% clindamycin as a positive control and the spray base as a negative control. Data were analyzed using the non-parametric Kruskal-Wallis test after normality testing indicated that the data were not normally distributed. The results showed that all formulations containing lemongrass essential oil produced inhibition zones against Staphylococcus aureus and were classified as having weak antibacterial activity, while the negative control did not show any antibacterial effect. The Kruskal–Wallis test showed no statistically significant difference among the formulations (p > 0.05); however, descriptively, Formula 3 showed the highest mean inhibition zone compared to the other formulations. In conclusion, the lemongrass essential oil-based foot cleansing spray shows potential as a natural antibacterial agent against Staphylococcus aureus, although further optimization of the active ingredient concentration and formulation system is needed to improve its antibacterial effectiveness.

Keanekaragaman Vegetasi Phanerogamae Berdasarkan Indeks Nilai Penting di Pulau Gili Iyang Sumenep-Madura

Argus Argus, Luluk Suhartini, Akhmad Ferdiansyah, Mahrus Ali, Ah. Zahir — Sat, 31 Jan 2026 00:00:00 +0000

Gili Iyang Island is part of a small island located east of Madura Island, specifically in Sumenep Regency. To date, Gili Iyang Island is known as the oxygen island. The high oxygen content is thought to be related to the vegetation on Gili Iyang Island. Therefore, the purpose of this study is to analyze the structure and dominance of Phanerogamae vegetation based on the Importance Value Index on Gili Iyang Island, Sumenep-Madura. The research station location was determined using purposive sampling, consisting of 7 observation stations, with each station having 3 observation plots determined using the transect method with data analysis using the Importance Value Index. Meanwhile, the results of the study showed that there were 78 species of Phanerogamae vegetation identified and analyzed using the Importance Value Index with varying values. The highest Importance Value Index was for the species Lannea coromandelica from the Anacardiaceae family, with an Importance Value Index of 32.487.

Struktur dan Komposisi Vegetasi Pohon Berdasarkan Ketinggian Kawasan Batuan Intrusif Gunung Tanggul Kabupaten Tulungagung

Desi Kartikasari, Muhammad Riszky Wahyu Pradana, Muhammad Kharis Sanjani — Sat, 31 Jan 2026 00:00:00 +0000

Mount Tanggul was formed by a diorite intrusion penetrating limestone, containing quartz, plagioclase, and feldspar minerals that present potential for economic, educational, and tourism utilization. The vegetation in this region serves as an essential biotic element that maintains ecosystem balance. This study aimed to analyze the structure and composition of tree vegetation in the intrusive rock area of Mount Tanggul. The research was conducted in Besuki District from August to September 2022 using a purposive sampling method at three elevation levels: 100, 200, and 300 meters above sea level. At each station, observations were carried out within a 60 m × 20 m transect. Vegetation data for the tree layer were collected using the line method, while pole, sapling, and seedling layers were examined using the line-plot method. Data analysis included the Important Value Index (IVI) and Shannon-Wiener Diversity Index (H′). A total of 21 species from 14 families were identified, with different dominant species at each site: Cocos sp. (Arecaceae) at Station 1, Albizia chinensis (Fabaceae) at Station 2, and Durio sp. (Malvaceae) together with Syzygium aromaticum (Myrtaceae) at Station 3. Diversity values (H′) showed a consistent increase from tree to seedling strata, with the lowest at tree level (H′ = 0.00–1.09) and the highest at seedling level (H′ = 1.62–1.72). These results indicate moderate diversity in regenerative strata but low diversity in the canopy due to species dominance. Vegetation stratification between strata C and B suggests that the community remains at an intermediate successional stage.

Struktur Komunitas Gastropoda pada Zona Intertidal di Pantai Motaain Desa Motaain Kecamatan Malaka Barat Kabupaten Malaka

Mersiana Hoar Bria, Blasius Atini, Maria Paulin Saridewi — Sat, 31 Jan 2026 00:00:00 +0000

This study aims to determine the structure of the gastropod community including type, diversity, uniformity, dominance, and distribution patterns in the intertidal zone of Motaain Beach, Motaain Village, West Malaka District, Malaka Regency. This study was conducted in March 2025 with data collection at one station. The transect method used in this study was a quadrat with plot placement on each transect line. Data analysis included diversity index, uniformity, dominance, and distribution patterns. Based on the results of the study, 7 species of gastropods from 5 families were obtained, namely Nerita lineata, Cerithidea quoyii, Letterend olive, Terebralia palustris, Canarium mutabile, Telescopium telescopium, Ellobium aurisjudae. The diversity (H’) of this station is categorized as moderate because the index value obtained at this station is 1.619 including the criteria 1 < H < 3. The uniformity (E) of this station is categorized as having high uniformity because the uniformity value obtained at this station is 0.83, including the criteria <0.00 (E). The dominance (C) of this station is categorized as low because the dominance value at this station is 0.21 including the criteria (C < 0.50). The distribution pattern (id) of this station is categorized as clustered because the distribution value at this station is 0.994 including the criteria ip > 0. The average value of environmental parameters is temperature 29℃, average salinity 30.5 ppt, average pH value 6.5 and current velocity 0.365 m/s.

Potensi Produk Bioteknologi Pangan dan Farmasi Berupa Proporsi Kombucha Daun Hanjeli (Coix lacryma-jobi) dan Bunga Telang (Clitoria ternatea L) Dalam Menangkal Radikal Bebas

Sat, 31 Jan 2026 00:00:00 +0000

This study aimed to evaluate the potential of kombucha-based biotechnology products derived from Job’s tears leaves (Coix lacryma-jobi) and butterfly pea flowers (Clitoria ternatea L.) in scavenging free radicals based on different formulation proportions. A completely randomized design was applied with seven treatment formulations of Job’s tears leaves and butterfly pea flowers, including a control, fermented for seven days. The parameters analyzed included pH, total acidity, total soluble solids (TSS), total microbial count, and antioxidant activity evaluated using the DPPH free radical scavenging method and expressed as IC₅₀ values. The results showed that all treatments experienced a decrease in pH (from 4.00–4.08 to 3.16–3.47) and TSS, along with an increase in total acidity and total microbial population during fermentation. Antioxidant activity significantly increased after fermentation, as indicated by a reduction in IC₅₀ values across all treatments, with final IC₅₀ values ranging from 143 to 188 ppm. The formulation containing 100% butterfly pea flower exhibited the highest antioxidant activity, with an IC₅₀ reduction of 51.73%, followed by the formulation containing 50% Job’s tears leaves and 50% butterfly pea flower, which showed a reduction of 47.47%.These findings demonstrate that raw material composition significantly influences fermentation characteristics and antioxidant capacity of kombucha. Kombucha formulated with butterfly pea flower and Job’s tears leaves shows strong potential as a functional food with high antioxidant activity and as a natural antioxidant raw material for pharmaceutical preparations, particularly for applications related to oxidative stress prevention.

Inventarisasi Jenis Bambu di KHDTK Tahura Sultan Adam Mandiangin, Kalimantan Selatan

Nur Azizah, Bunda Halang — Sat, 31 Jan 2026 00:00:00 +0000

Bamboo (Poaceae) played an important ecological and economic role in tropical regions, including Indonesia. As a Special Purpose Forest Area (KHDTK), Tahura Sultan Adam Mandiangin served a strategic role in education, research, and forest resource management; however, the availability of scientific data on bamboo diversity in this area remained limited. This study aimed to inventory and describe the bamboo species in KHDTK Tahura Sultan Adam Mandiangin to provide a baseline for forest management and the development of biology learning resources. The study employed a descriptive-explorative approach with a purposive sampling technique. Observations focused on open habitats and riverbanks at an elevation of 74–75 m above sea level, considered potential bamboo growth habitats. Species identification was conducted based on vegetative morphological characters, including culms, sheaths, branching, and leaves. The results showed eight bamboo species from five genera, with Bambusa vulgaris as the most dominant and widely distributed species. The dominance of the genus Bambusa reflected the ecosystem characteristics of the area, which were dominated by open and riparian zones. These findings provided essential baseline data to support KHDTK functions in conservation and the enhancement of biology learning resources based on local potential.

Evaluasi Kadar Lipid Ikan Zebra (Danio rerio Hamilton, 1822) Model Hiperlipidemia Setelah Perlakuan Ekstrak Akuosa Daun Sirsak (Annona muricata L.)

Sat, 31 Jan 2026 00:00:00 +0000

Zebrafish are often chosen as an animal model because of their high physiological similarity to humans, their rapid life cycle, and their transparent embryos that facilitate direct visualization of organ development, making them an effective animal model for screening the effectiveness of natural agents and understanding disease mechanisms. This study aimed to evaluate the antihyperlipidemic effect of soursop leaf aqueous extract (Annona muricata L.) on hyperlipidemic animal model zebrafish (Danio rerio Hamilton, 1822). Hyperlipidemic conditions were obtained by induction using a 0.1% quail egg yolk suspension for 48 hours in zebrafish aged 5 days post fertilization. The concentration of extract was determined based on the MNLC (Maximum Non-Lethal Concentration) method. Based on the results of the MNLC test, the determined extract concentrations were 1/10 MNLC, 1/3 MNLC, and MNLC. The antihyperlipidemic effect was evaluated based on the measurement of total triglycerides and total cholesterol using spectrophotometric method and the results of Oil Red O (ORO) staining. The results of the spectrophotometric test showed that the most effective concentration of soursop leaf aqueous extract to reduce triglyceride and cholesterol levels in zebrafish larvae was the MNLC concentration. Soursop leaf aqueous extract with MNLC concentration significantly (p>0.05) reduced triglyceride and total cholesterol levels in zebrafish by 40.52 and 71.54%, respectively. Spectrophotometric measurements also positively correlated with the results of ORO staining, which showed a decrease in red color intensity. The results of this study indicate that soursop leaf aqueous extract has the potential to have antihyperlipidemic properties and can be studied in animal model zebrafish.