PAPARAN 28 HARI EKSTRAK METANOLIK Scurrula atropurpurea TERHADAP KADAR SGPT TIKUS BETINA
Article Sidebar
Main Article Content
Abstract
Tea parasite Scurrulaatropurpurea (Bl.) Dans contains several secondary metabolites, one of which is Flavonoids from Quersetin that may act as antioxidants; the presence of antioxidants can neutralize and protect the liver from free radicals. Liver cell damage can be seen from the results of clinical biochemical test form levels of Serum Glutamic Pyrufic Transaminase (SGPT). The purpose of this study was to determine the effect of methanolic extract Scurrulaatropurpurea (MESA) to the SGPT levels of  female white mice for 28 days. Data were analysed using ANOVA in SPSS version 17.0. The numbers of tested animals were 20 female white mice which was divided rat into four groups, each group contained 5 mice. Group 1 is a dick, group 2, 3 and 4 as a treatment.  A significant difference between the mean treatment groups compared with the control group. No significant value difference between the groups was p> 0.05. Therefore, EMSA that was given to female mice for 28 days with a dose of 250 mg / KgBW, 500 mg / KgBW and 1000 mg / KgBW in the treatment group all the doses were not significantly different compared with the controls, in this case the level of SGPT female white mice was normal.
Benalu teh (Scurrula atropurpurea (Bl.) Dans mengandung beberapa senyawa metabolit sekunder salah satunya yaitu Flavonoid dari Quersetin yang berperan sebagai antioksidan. Keberadaan antioksidan mampu menetralkan dan melindungi hati dari radikal bebas. Kerusakan sel hati dapat dilihat dari hasil pemeriksaan biokimia klinis berupa kadar Serum Glutamic Pyrufic Transaminase (SGPT). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian Ekstrak Metanolik Scurrula atropurpurea (EMSA) terhadap kadar SGPT pada tikus putih betina selama 28 hari. Jenis penelitian ini adalah penelitian eksperimental dan analisis data menggunakan uji ANOVA dengan SPSS versi 17.0. Jumlah hewan uji adalah 20 ekor tikus putih betina dibagi menjadi 4 kelompok, masing-masing kelompok terdapat 5 ekor tikus. Perbedaan signifikan antara rata-rata kelompok perlakuan dibandingkan dengan kelompok kontrol. Berdasarkan hasil penelitian menunjukkan bahwa tidak terdapat perbedaan  yang signifikan antara semua kelompok dengan nilai  p>0.05. Maka dari itu EMSA yang diberikan kepada tikus betina selama 28 hari dengan dosis 250 mg/KgBB, 500 mg/KgBB dan 1000 mg/KgBB pada kelompok perlakuan semua dosis tidak berbeda nyata dibandingkan dengan kontrol, dalam hal ini kadar SGPT pada tikus putih betina bersifat normal.Â
Article Details
Copyright and Attribution:
Jurnal Biosaintropis (Bioscience-Tropici s licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC-BY). The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to automatic transfer of the copyright to the publisher.
Permissions:
Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from one of the authors.
Ethical matters:
Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the authors™ own must have had the correct authorization for this exportation.
References
[2] Athiroh, N. 2009. Kontraktilitas Pembuluh Darah Arteri Ekor Tikus Terpisah Dengan atau Tanpa Endotel Setelah Pemberian Esktrak Scurulla oortiana (Benalu Teh). Jurnal Berkala Hayati Edisi Khusus 3D. 31-34.
[3] Athiroh, N and E. Sulistyowati, 2013. Scurrula atropurpurea increases Nitric Oxide and decreases Malondialdehyde in hypertensive rats. Univ Med., 32: 44e50.
[4] Athiroh, N., N. Permatasari, D. Sargowo and M.A. Widodo, 2014. Antioxidative and Blood Pressure-Lowering Effects from Scurrula atropurpurea on DOCA-salt Hypertensive Rats. Biomarkers and Genomic Medicine. 6(1): (32-36).
[5] Athiroh, N., Permatasari, D. Sargowo and M.A. Widodo, 2014. Effect of Scurrula atropurpurea on Nitric Oxide, Endothelial Damage, and Endothelial Progenitor Cells of DOCA-salt Hypertensive Rats. Iran J Basic Med Sci., 17: 622-625
[6] Silalahi, jansen. 2002. Senyawa polifenol sebagai komponen aktif yang berhasiat dalam teh. Majalah kedokteran Indonesia 52 no 10. Hal: 361-4.
[7] Danbar S, Bose A, Chattaraj T K, Pal T K. 2009. Protective Role Of Zingiber Officinale Roscoe On Aceclofenac Induced Oxidative Stress In Rat Liver. International Journal of pharm Tech Research. 2:495-501.
[8] Khotimah, Siti. 2005. Pengaruh pemberian Ekstrak Jinten Hitam Terhadap Kadar GSH paru dan GSH Hepar Tikus Wistar yang Di Papar Asap Rokok. Penelitian Eksperimental Laboratorium. Program Paskasarjana UNAIR. Surabaya.
[9] Girindra, A. 1988. Biokimia I. PT.Gramedia Pustaka Utama. Jakarta.
[10] Suarsana dan Budiasa. 2005. Potensi Hepatoproteksi Ekstrak Mengkudu pada Keracunan Parasetamol. Jurnal Penelitian Kedokteran.
[11] Athiroh, N and Sulistiyowati E. 2015. Evalution of Methanolic Extract of Scurrulla atropurpurea (Bl.) Dans Sub-Chronic Exposure on Wistar Rat Liver. AENSI Journals.ISSN-1995-0756.
[12] Amiruddin R. 2006. Fisiologi dan Biokimia Hati. Dalam: Buku Ajar Ilmu Penyakit Dalam. Jilid I Edisi IV. Balai Penerbit FKUI. Jakarta. Hal : 417.
[13] Sodikin. 2002. Biokimia Enzim. Widya medika. Jakarta.
[14] Athiroh and Permatasari, 2012. Mechanism of Tea Mistletoe Action on Blood Vessels Medical Journal Brawijaya., 27(1): 1-4.
[15] Girindra, A. 1988. Biokimia I.: PT.Gramedia Pustaka Utama. Jakarta.
[16] Janbaz KH, Saeed SA, Gilani AH. 2004. Studies on the protective effects of caffeic acid and quercetin on chemical-induced hepatotoxicity in rodents. Phytomedicine: (11) 424-430.
[17] BPOM. 2014. Pedoman Uji Toksisitas Nonklinik secara in vivo. BPOM. Jakarta.