The Effect of Exposure of Cigarette Smoke With Herb Additives on Leukocyte and Lung Histopathology of Mice (<i>Mus musculus</i>)
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Abstract
Smoking habits have been around since ancient times, but nowadays this habit is considered to be detrimental, especially to health. The impact that is often felt by smokers is difficulty in breathing because the lungs are exposed to cigarette smoke. Cigarette smoke contains about 1015-1017 oxidants or free radicals, as well as 4700 harmful chemicals, including aldehydes / carbonyls, NO2, and SO2. Herbal cigarettes are tobacco cigarettes with added ingredients from plants. Gurah terapi sin cigarettes are herbal cigarettes that are sold commercially. The aim of this study was to determine the effect of gurah cigarette smoking on the leukocytes and lung histology of mice. This study used a comparative method consisting of 3 groups, namely the control was not exposed to cigarette smoke, treatment 1 was exposed to commercial cigarette smoke and treatment 2 was exposed to cigarette smoke with herbal ingredients and each group consisted of 10 replications. The results showed that there were significant differences (p <0.05) regarding the number of cell necrosis, type II pneumocytes, inflammatory cell infiltration, hemorrhage, and alveolar dilation. While the results of the analysis of the number of leukocytes showed no significant difference where p > 0.05. The results showed that there was no significant difference in the number of leukocytes in the control group, treatment 1 and treatment 2 (p > 0.05). herbs containing various kinds of antioxidants cause a tendency for differences in the number of leukocytes where there is a decrease in the number of lymphocytes and neutrophils and an improvement in the histopathological structure of the lung against type I pneumocyte cell necrosis, hemorrhage, alveolar dilation, type II pneumocyte cell proliferation, and inflammatory cell infiltration in exposed mice. commercial cigarette smoke without herbal ingredients.
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References
[2] Baker, R. R. 2006. Smoke Generation Inside a Burning cigarette : Modifying Combustion to Develop Cigarettes That May be Less Hazardous to Health. 375-385
[3] Angelis, N., K. Propodis., P. Zarogoulidis., D. Spyratos., I. Kioumis., A. Papaiwannouw and G. Pitsiouw. 2014. Airway Inflammation in Chronic Obstructive Pulmonary Disease. Journal Thorac. Dis. 6 : 4-9
[4] Fitriani., K. Eriani., W. Sari. 2009. The effect of cigarettes smoke exposured causes fertility of male mice (Mus musculus). Jurnal Natural. 10(2) : 1-6.
[5] Lin, B., X. Li., H. Zhang., Z. Lin., L. Tian., C. Nie and Z. Xi. 2014. Comparison of In Vitro Toxicity of Mainstream Cigarette Smoke Particulate Matter From Nano- to Micro- Size. Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association. 64 : 353-360
[6] Valvanidis, A., T. Vlachogiani and K. Fiotakis. 2009. Tobacco Smoke: Involvement of Reactive Oxygen Species and Stable Free Radicals in Mechanisms of Oxidative Damage, Carcinogenesis and Synergistic Effects with Other Respirable Particles. International Journal of Enviromental Research and Public Health. 6(2) : 445-462. DOI: 10.3390/ijerph6020445
[7] Daher, N., R. Saleh, E. Jaroudi, H. Sheheitli, T. r. s. Badr, E. Sepetdjian, M. A. Rashidi, N. Saliba dan A. Shihadeh. 2009. Comparison of carcinogen, carbon monoxide, and ultrafine particle emissions from narghile waterpipe and cigarette smoking: Sidestream smoke measurements and assessment of secondhand smoke emission factors. Atmospheric Environment: 1-2. DOI: 10.1016/j.atmosenv
[8] Smith, K.R., K.E. Pinkerton., T. Watanabe., T.L. Pedersen., S.J. Ma and B.D. Hammock. 2005. Attenuation of Tobacco Smoke-Include Lung Inflammation by Treatment With a Soluble Epoxide Hydrolase Inhibitor. Journal Pharmacology. 102(6) : 2186 – 2191. DOI: 10.1073/pnas.0409591102
[9] Yu, L., B. G. Dzikovski dan J. H. Freed. 2012. A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke. 1-5. DOI: 10.3791/3406
[10] Zhang, D., Y. Tao., J. Gao., C. Zhang., S. Wan., Y. Chen., X. Huang., X. Sun., S. Duan., F. Schonlau., P. Rohdewald and B. Zhao. 2002. Pycnogenol In Cigarette Filters Scavenger Free Radicals and Reduce Mutagenicity and Toxicity of Tobacco Smoke in Vivo. Toxicology and Industrial Health. 18(5) : 215-224. DOI: 10.1191/0748233702th145oa
[11] Sukmaningsih, A.A.S.A., S. Permana., D.J.D.H. Santjojo., A.Y.P. Wardoyo dan S.B. Sumitro. 2019. The Potency of Java Plum (Syzygium cumini) Fruit Extract as Free Radical Scavenging in Cigarette Smoke. AIP Conference Proceeding. 1-6. DOI: 10.1063/1.5125519
[12] Muntiha, M. 2001. Teknik Pembuatan Preparat Histopatologi dari Jaringan Hewan dengan Pewarnaan Hematoksilin dan Eosin. Balai Penelitian Veteriner. Bogor.
[13] Katherine N., G.Corley, K.O. Alicia, and D.K. Meyerholz. 2013. Principles For Valid Histopathologic Scoring In Research. Vet Pathol. 50(6)
[14] Barasi, M. E. 2009. At a Glance Ilmu Gizi (H. Halim, Trans). Erlangga Medical Series. Jakarta
[15] Malenica, M., B. Prnjavorac., T. Bego., T. Dujic., S. Semiz., S. Skrbo., AGusic., A. Hadzic and A. Causevic. 2017. Effect of Cigarette Smoking on Haematological Parameters in Healthy Population. Medical Archives. 71(2) : 132-136 DOI: 10.5455/medarh.2017.71.132-136
[16] Arnson, Y., Y. Shoenfeld and H. Amital. 2010. Effect of Tobacco Smoke on Immunity, Inflamation and Autoimmunity. Journal of Autoimmunity. 34(3) : 258-265 DOI: 10.1016/j.jaut.2009.12.003
[17] Maulana, I., H. I. Wahyuni dan T. Yudiarti. 2019. Pengaruh Penambahan Ekstrak Tomat Sebagai Air Minum Terhadap Profil Darah Putih Ayam Broiler yang Diinfeksi Bakteri E. coli. Jurnal UNS. 3(1) : 34-41
[18] Kania, N. 2014. Pathobiologi Molekuler Paru. PT. Grafika Wangi Kalimantan. Kalimantan
[19] Holgate, S.T., P. Lackie., S. Wilson., W. Roche and D. Davies. 2000. Bronchial epithelium as a key regulator of airway allergen sensitization and remodeling in asthma. Am J Respir Crit Care Med. 162. DOI: 10.1164/ajrccm.162.supplement_2.ras-12
[20] Sela, S., Shurtz-Swirski, R., Awad, J., Shapiro, G., Nasser, L and S.M. Shasha. 2004. The Involvement of Peripheral Polymorphonuclear Leukocytes in the Oxidative Stress and Inflammation among Cigarette Smokers. Israel Medical Association Journal. 4(11) : 1015-1019. DOI: 10.1681/ASN.2004110929
[21] Sukmaningsih, A.A.S.A, S. Permana., D.J.D.H. Santjojo., A.Y.P. Wardoyo dan S.B. Sumitro. 2018. Investigating Natural Transition Metal Coordination Anthocyanin Complex In Java Plum (Syzygium cumini) Fruit as Free Radical Scavenging. Rasayan J. Chem. 11(3) : 1193-1203. DOI : 10.31788/RJC.2018.1133047
[22] Sun, Y., S. Ito., N. Nishio., Y. Tanaka., N. Chen and K.I. Isobe. 2014. Acrolein induced both pulmonary inflammation and the death of lung epithelial cells. Toxicology Letters. 229(2) : 384-392. DOI: 10.1016/j.toxlet.2014.06.021
[23] Mitchell, R.N., V. Kumar., A.K. Abbas and N. Fausto. 2009. Antioxidant Activity of Medical and Aromatic Plants a Review. Flavour Fragr Journal. 25(29) : 291-312
[24] Khaira, K. 2010. Menangkal Radikal Bebas Dengan Anti-oksidan. Jurnal Saintek. 2(2) : 183-187
[25] Junqueira, Luis.C and J. Carneiro. 2009. Histologi Dasar. Edisi 10. EGC. Jakarta
[26] Honda T, Ota H, Yamazaki Y, Yoshizawa A, Fujimoto K, and Sone S. 2003. Proliferation of type II pneumocytes in the lung biopsy specimens reflecting alveolar damage. Respir Med. 97(1): 80-85. DOI: 10.1053/rmed.2002.1408
[27] Suryadinata, R.V. 2018. Pengaruh Radikal Bebas Terhadap Proses Inflamasi pada Penyakit Paru Obstruktif Kronis (PPOK). Jurnal Sains. 2(1) : 37-324 DOI: 10.20473/amnt.v2i4.2018.317-324
[28] Wynimko, J.C. 2016. Disease modification in emphysema related to alpha-1 antitrypsin deficiency. Taylor and Francis Group. 1(1) : 1-9. DOI: 10.1080/15412555.2016.1178224
[29] Rahmawandani, F.I. 2013. Studi Patologi Kasus Kolibasilosis Pada Babi Landrace Berdasarkan Umur. Denpsar. FKH Universitas Udayana: [Skripsi].
[30] Masruroh, L., U. P. Juswono dan A. Y. P. Wardoyo. 2014. Pengaruh Emisi Partikel Ultrafine Asap Pembakaran Biomassa Terhadap Organ Paru-Paru Mencit (Mus musculus) Berdasarkan Gambaran Mikroskopisnya. Brawijawa Physics Student Journal. 1-4
[31] Herdman, H. 2010. Diagnosa keperawatan. EGC. Jakarta
[32] Nurjannah, D.A., R. Retnowati dan U. P. Juswono. 2013. Aktivitas Antioksidan dari Minyak Cengkeh (Syzygium aromaticum) Kering Berdasarkan Aktivitas Antiradikal yang Ditentukan Mengguanakan Electron Spin Resonance. Kimia Student Journal. 1(2) : 283-288
[33] Qonitah, F dan Ahwan. 2018. Aktivitas Antioksidan dan Kandungan Fenolik Total dari Isolat Polar Fraksi Heksana Ekstrak Etanol Daun Sirih (Piper betle L.). Jurnal Farmasetis. 7(1) : 42-46
[34] Zarai, Z., E. Boujelbene., N.B. Salem., Y. Gargouri and A. Sayari. 2012. Antioxidant and antimicrobial activities of various solvent extracts, piperinae and piperic acid from Piper nigrum. LWT - Food Sci Technol. 50(2):634-641.