Main Article Content
As a maritime country with vast waters, Indonesia has many opportunities to utilize marine resources as a source of bioactive compounds that have the potential as active medicinal ingredients. One of the marine biotas that potentially contains the active compounds is the Vannamei shrimp's shell (Litopenaeus vannamei), which is commonly found as waste along the coast of Gunungkidul, Yogyakarta. The shrimp’s shell contains astaxanthin, a potential source of antioxidants for the health industry. The purpose of this study was to compare the astaxanthin extraction yield from L. vannamei shrimp shells using sunflower oil and 70% ethanol. The Astaxanthin extraction used sunflower oil and ethanol 70% as solvents and was done by maceration method, while the phytochemical test and Astaxanthin profiling used Thin Layer Chromatography and Spectrophotometer with Kelly and Harmon (1972)  calculations as well as pure Astaxanthin standards. The extraction yield of the 70% ethanol extraction was further processed by column chromatography using ether: ethanol (8: 2) as mobile phase. The highest Astaxanthin yield (220 mg / g of shrimp powder) was obtained from the extraction with sunflower oil compared to the 70% ethanol solvent, while the fractionation result with a chromatographic column from a crude extract of ethanol 70% showed high astaxanthin yield of 220.77 mg. / g fraction. The results of the fraction test on rat neutrophils, the best percentage reduction was at a concentration of 150 mg / g bw of rats.
This work is licensed under a Creative Commons Attribution 4.0 International License.
 Davinelli S., Nielsen E. M., Scapagnini G. (2018). Astaxanthin in Skin Health, Repair, and Disease: A Comprehensive Review. Nutrients,10:4 522; doi:10.3390/nu10040522.
 Dalei J. and D. Sahoo. (2014). Extraction And Characterization Of Astaxanthin From The Crustacean Shell Waste From Shrimp Processing Industries. IJPSR, 2015; Vol. 6(6): 2532-2537.
 Guerin, M., M. E. Huntley, dan M. Olaizola. 2003. Haematococcus astaxanthin: applications for human health and nutrition. Trends in Biotechnology. 21(5) : 210–216
 Kelley, C.E.; Harmon, A.W, 1972. Method of Determining Carotenoid Contents Of Alaska Pink Shrimp And Representative Values For Several Shrimp Products. Fish. Bull., 11-17.
 Kobayashi, M. and Sakamoto, Y. 1999. Singlet oxygen quenching ability of astaxanthin esters from the green algae Haematococcus pluvialis. Biotechnology Letters, 21: 265–269
 Kuedo Z., A. Sangsuriyawong, W. Klaypradit, V. Tipmanee, P. Chonpathompikunlert. 2016. Effects of Astaxanthin from Litopenaeus Vannamei on Carrageenan-Induced Edema and Pain Behavior in Mice. J. Molecules. Mar; 21(3): 382. Published online 2016 Mar 19. doi: 10.3390/molecules21030382
 Lorenz T. R., 1998. Thin Layer Chromatography (TLC) System For Natu Rose Carotenoids. Technol. Bulletin 003: 1-3
 Masir O., Menkher M., Andani E.P., Salmiah A. 2012. Pengaruh Cairan Cultur Filtrate Fibroblast (CFF) Terhadap Penyembuhan Luka; Penelitian eksperimental pada Rattus Norvegicus Galur Wistar. Jurnal Kesehatan Andalas DOI: 10.25077/jka.v1i3.78
 Sachindra, N. M., & Mahendrakar, N. S. (2005). Process optimization for extraction of carotenoids from shrimp waste with vegetable oils. Bioresource Technology, 96(10), 1195- 1200
 Stamatios P., Thomais V., Athanasios V. 2013. Bioactive Natural Substances from Marine Sponges: New Developments and Prospects for Future Pharmaceuticals. University of Athens, Greece.
 Swastawati F., I. Wijayanti, E. Susanto (2008). Pemanfaatan Limbah Kulit Udang Menjadi Edible Coating untuk Mengurangi Pencemaran Lingkungan.puslit2.petra.ac.id/ ejournal/index.php/jtl/article/viewFile/17554/17469.4:101-106