Pengaruh Fraksi Ejakulasi terhadap Motilitas Spermatozoa Kambing Peranakan Etawa (Capra aegagrus) The Effect of Ejaculation Fraction on the Spermatozoa Motility of Etawa Crossbred Goat (Capra aegagrus)
Article Sidebar
Main Article Content
Abstract
The ejaculation fraction on Etawa crossbred goat (PE goat) has various standard spermatozoa qualitues, one of which ismotility of spermatozoa. The motility of spermatozoa is very important to note in the development of artificial insemination techniques, because the success in artificial insemination techniques is strongly influenced by the motility of spermatozoa. The motility of semen spermatozoa is known through the process of examining the movement of spermatozoa in microscopic testing. The aim of this research was find out the effect of ejaculation fraction on motility of PE goat spermatozoa. Using an experimental method with a group's random consisting of two treatment groups; K1 is fraction 1and K2 is a fraction 2. The Replication of each treatment was eight replications and a total of sample research was 16 unit. The semen was collected once a week using artificial vagina (VA) technique. The semen collecting until the second ejaculation and motility test of spermatozoa were immediately measured use sperm vision analyzers IVOS II. The test results of the ejaculation fraction observation using an independent sample t-test showed the value of t hit = 0.700 and t (0.025) than 2.14 t hit <t (0.025) meaning that H0 was received, there was no significant ejaculation fraction 1 with ejaculatory fraction 2; motility meaning of the spermatozoa from the PE goat of a fraction 1 and faction 2 not different significantly.
Keywords: ejaculation fraction, motility of Spermatozoa, PE goat.
ABSTRAK
Fraksi Ejakulasi pada Kambing Peranakan Etawa (PE) mempunyai kualitas spermatozoa dengan berbagai standart, salah satunya motilitas spermatozoa. Motilitas Spermatozoa menjadi hal yang sangat penting untuk diperhatikan dalam pengembangan teknik Inseminasi Buatan, karena keberhasilan dalam teknik Inseminasi Buatan sangat dipengaruhi oleh motilitas yang dimiliki spermatozoa. Motilitas spermatozoa semen diketahui melalui proses pemeriksaan daya gerak spermatozoa dalam uji mikroskopis. Penelitian ini bertujuan untuk mengetahui pengaruh fraksi ejakulasi terhadap motilitas spermatozoa kambing PE. Menggunakan metode percobaan dengan rancangan acak kelompok (RAK) terdiri atas 2 kelompok perlakuan yakni Kelompok 1 (K1) adalah fraksi 1dan kelompok 2 (K2) adalah fraksi 2, ulangan masing-masing perlakuan 8 ulangan dan unit penelitian berjumlah 16. Penampungan semen dilakukan satu kali seminggu dengan teknik AV (Artificial Vagina). Penampungan dilakukan sampai ejakulat kedua. Segera setelah penampungan dilakukan pengukuran uji motilitas spermatozoa menggunakan sperm vision analyser IVOS II. Hasil uji pengamatan fraksi ejakulasi menggunakan t-test independent sample menunjukkan nilai t hit= 0,700 dan t (0,025)= 2,14 maka t hit < t (0,025) artinya H0 di terima, yang mempunyai arti motilitas spermatozoa kambing PE dari fraksi 1 dan fraksi 2 tidak berbeda nyata.
Kata kunci: fraksi ejakulasi, motilitas spermatozoa, kambing PE
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Attribution:
Jurnal Biosaintropis (Bioscience-Tropici s licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC-BY). The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to automatic transfer of the copyright to the publisher.
Permissions:
Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from one of the authors.
Ethical matters:
Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the authors™ own must have had the correct authorization for this exportation.
References
[2] Heriyanta, E., Nur I, Dan Isnaini N. 2013. Pengaruh Umur Kambing Peranakan Etawah (Pe) Terhadap Kualitas Semen Segar. J. Ternak Tropika Vol. 14, No.2: 1-5.
[3] Batubara, A., Nasution, S., Subanriyo, 2016. Kambing Peranakan Etawa (PE). Badan Penelitian dan Pengembangan Pertanian IAARD Press. Jakarta.
[4] Hafez, E.S.E. 2000. Semen Evaluation. in Reproduction in Farm Animals. 7th ed. Lea and Febiger, Philadelphia.
[5] Toelihere, M. R. 1993. Inseminasi Buatan Pada Ternak. Angkasa. Bandung.
[6] Feredis. 2010. Bioteknologi Reproduksi pada Ternak. Alfabeta . Bandung.
[7] Setiadi, B., Subandriyo, K. Dwiyanto, T. Sartika, B. Tiesnamurti, U.D. Yulistiani dan Martawidjadja, M. 2000. Karakterisasi Sumberdaya Genetik Kambing Lokal sebagai Upaya Pelestarian Secara Ex-Situ. Balai Penelitian Ternak, Ciawi, Bogor.
[8] Toelihere M. R. 1985. Inseminasi Buatan pada Ternak. Angkasa. Bandung.
[9] Kaya, A., Varsakeli, S., and Karatzas, F., 2000. Characteristics and Seasonal Variation the semen of Alphine, Saanen and Damascus goat bucks born and raised in Greece. Theriogenology. 53: 1285-1293.
[10] Susilawati, T., Suyadi, Nuryadi, N. Isnaini dan S. Wahyuningsih. 1993. Kualitas Semen Sapi Fries Holland dan Sapi Bali pada Berbagai Umur dan Berat Badan. Laporan Penelitian. Fakultas Peternakan Universitas Brawijaya, Malang.
[11] Evans. G. and Maxwell, W.M.C. 1987. Salamon’s Artificial Insemination of Sheep and Goat. Butterworth. London
[12] Zahraddeen, D., Butswat I.S.R., Kalla, D.J.U, Sir, S.M., Bukar, M.T. 2005. Effect of Frequency of Ejaculation on Semen Characteristics in Two Breeds of Turkeys (Meleagris gallopavo) Raised in a Tropical Environment. Int. J. Poult. Sci. 4(4): 217-221.
[13] Mostari, M.P., Rahman, M.G.M., Khandoker, M.A.M.Y. and Husain, S.S. 2004. Evaluation of bulls based on semen quality and herd fertility.
[14] Toelihere, M R. 1981. Fisiologi Reproduksi pada Ternak. Angkasa. Bandung.