Pengaruh Ekstrak Metanolik (Scurrula atropurpurea (Bl.) Dans) Yang Diberikan Secara Subkronik 90 Hari Pada Tikus Betina (Rattus norvegicus) Terhadap Necrosis Otak (Metanolic Extraction of (Scurrula atropurpurea (Bl.) Dans) Effect which is given 90-Days Sub-chronic on Female Rats (Rattus norvegicus) toward Necrosis of Brain )
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Abstract
The goals of this research is to know the effect of MESA (Extraction of Methanolic Scurrula atropurpurea (Bl.) Dans toward the description of necrosis of brain female rats (Rattus norvegicus) by sub-chronic 90 days. The method of this research uses True Experimental Design by complete randomized design. The samples which are used are 40 rats that divided into 4 treatments, a control group (without exposed by MESA) 3 treatment groups are exposed by multilevel MESA those are 250 mg/Kg BW, 500 mg/KgBW and 1000 mg/KgBW. In the 90th day, the rats are sacrificed, the rats are anesthesia then the rats are observed by macro-pathology that will be pondered (absolute weight) must be dried with absorbent paper. Meanwhile the analyzed data is relative weight that is absolute organs weight divided by body weight. Organs examined histopathologies which are observed are brain organs. Every organs and tissues were separated, entered into formaldehyde solution 10% and made histopathology preparations then examined under microscope. The result of this research is shown that giving MESA between groups of treatment compared to group of control are insignifican. means is not getting toxic toward female rats. Due the active substances contained in MESA is Flavonoids and Kuersetin that contain antioxidans and can inhibit free radicals.
Keyword: Brain, Necrosis, Mistle Toe
ABSTRAK
Tujuan dari penelitian ini adalah untuk mengetahui pengaruh EMSA (Ekstrak Metanolik Scurrula atropurpurea (BI.) Dans) terhadap gambaran nekrosis otak tikus (Rattus norvegicus) betina secara subkronik 90 hari. Metode penelitian ini menggunakan True Experimental Design dengan Rancangan Acak Lengkap (RAL). Sampel yang digunakan sebanyak 40 ekor tikus. dengan dibagi 4 perlakuan, 1 kelompok kontrol (tanpa diberi EMSA) 3 kelompok perlakuan dengan diberi EMSA yang bertingkat yaitu 250 mg/KgBB, 500 mg/KgBB dan 1000 mg/KgBB. Pada hari ke-90 tikus dikorbankan, tikus dibius kemudian dilakukan pengamatan secara makropatologi yang akan ditimbang (bobot absolut) harus dikeringkan terlebih dahulu dengan kertas penyerap. Sedangkan data yang di analisis adalah bobot relatif, yaitu bobot organ absolut dibagi bobot badan. Organ yang diperiksa histopatologi yang diamati adalah organ otak. Setiap organ dan jaringan yang sudah dipisahkan dimasukkan dalam larutann formalin 10% dan dibuat preparat histopatologi kemudian di periksa dibawah mikroskop. Hasil penelitian ini menunjukan bahwa pemberian EMSA pada perlakuan tikus kontrol (tanpa perlakuan) tidak berbeda nyata dibandingkan dengan tikus perlakuan, sehingga EMSA aman (tidak toksik ) terhadap tikus betina dikarenakan zat aktif yang terkandung dalam EMSA.
Kata kunci: Otak, Nekrosis, Benalu Teh
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